Fig. 6: Effects of DR-Ab on the internalization of NKAα1 and GluR2.
From: DR-region of Na+/K+ ATPase is a target to treat excitotoxicity and stroke
a Representative blots of co-immunoprecipitation assay showing direct interaction between GluR2 and NKAα1. Cell lysates were immunoprecipitated with anti-GluR2 and anti-NKAα1 antibodies and blotted with anti-NKAα1 and anti-GluR2 antibodies respectively. b, c Western blots analysis showing that DR-Ab reversed membrane loss of NKAα1 (b), n = 6/group, and GluR2 (c), n = 5/group, upon glutamate treatment. One-way ANOVA followed by Bonferroni’s test, *p < 0.05, **p < 0.01. DR: DR-Ab; Glu: glutamate (100 µM) + glycine (10 μΜ) for 30 min. NS: IgG purified from normal rat serum. d Western blots showing that the plasma membrane protein level of GluR2 was significantly reduced in NKAα1+/− mice, n = 4/group. Unpaired t test, two-tailed, *p < 0.05 vs NKAα1+/+ mice. M-NKAα1: membrane NKAα1; T-NKAα1: total NKAα1
