Fig. 7: CCN3 restrains osteogenic differentiation of MEFs by inhibiting Hey1. | Cell Death & Disease

Fig. 7: CCN3 restrains osteogenic differentiation of MEFs by inhibiting Hey1.

From: CCN3 and DLL1 co-regulate osteogenic differentiation of mouse embryonic fibroblasts in a Hey1-dependent manner

Fig. 7

a–c qRT-PCR was employed to measure the effects of CCN3 (a), siCCN3 (b), and DLL1 (c) on the mRNA level of Hey1 at 3 days posttreatment. d, e ALP staining assay (d) and quantitative assay (e) to determine the ALP activity under the treatment as shown at 7 days postinfection (magnification ×100). The data are shown as mean ± SD for three separate experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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