Fig. 8: Analysis of FOXM1 binding on human TYMS promoter | Cell Death & Disease

Fig. 8: Analysis of FOXM1 binding on human TYMS promoter

From: RETRACTED ARTICLE: FOXM1 modulates 5-fluorouracil sensitivity in cholangiocarcinoma through thymidylate synthase (TYMS): implications of FOXM1–TYMS axis uncoupling in 5-FU resistance

Fig. 8

a FOXM1-binding site on human TYMS promoter. ENCODE (the Encyclopedia of DNA Elements) project ChIP sequencing data of FOXM1 binding in the MCF-7, SKSH and ECC1 cells were used for predicting global genome-binding profiles for FOXM1 using the Integrative Genomics Viewer (Version 2.3.88) and the hg19 UCSC Genome Browser50. The predicted binding profiles of FOXM1 and the locations of the designed ChIP primer pairs are aligned to the human TYMS promoter. b KKU-D131 and HuCCA cells treated with 100 nM 5-FU for 0, 24 and 48 h were used for chromatin immunoprecipitation assays using the IgG as negative control and anti-FOXM1 antibody. After reversal of cross-linking, the co-immunoprecipitated DNA was amplified by qRT–PCR, using primers amplifying the FOXM1 binding-site-containing region. Data are presented as mean ± SEM (n = 4, each with >3 replicates) and were analysed by two-way ANOVA (Supplementary Fig. S7). The asterisk (*) indicates significant difference at p < 0.05 and ‘ns’ no significant difference

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