Fig. 7: Nuclear accumulation of the p62-based ALIS is required for oxidative stress-induced parthanatos. | Cell Death & Disease

Fig. 7: Nuclear accumulation of the p62-based ALIS is required for oxidative stress-induced parthanatos.

From: Nuclear-accumulated SQSTM1/p62-based ALIS act as microdomains sensing cellular stresses and triggering oxidative stress-induced parthanatos

Fig. 7

a HT1080 cells were treated with 1 mg/ml cefotaxime for 36 h, and then performed immunofluorescence staining with ubiquitin antibody, and 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining. Arrows indicate the ALIS-containing nucleus. Scale bar, 10 μm. b, c HT1080 cells were treated with 1 mg/ml cefotaxime (b) or 0.4 mM H2O2 (c) for the indicated periods, and then the nuclear and cytoplasmic extracts were subjected to immunoblotting with the indicated antibodies. d, g HT1080 cells were treated with 1 mg/ml cefotaxime with or without 1 mM NAC (d) or 5 mM salicylate (g) for 30 h, and then cell extracts were subjected to immunoblotting with the indicated antibodies. e HT1080 cells were treated with 1 mg/ml cefotaxime for the indicated periods, and then cell extracts were subjected to immunoblotting under reduced or non-reduced condition. f p62 knockout HT1080 cells were transfected with Flag-p62 wild-type (WT) or Flag-p62 C105A/C113A (2CA) plasmid for 24 h, and then treated with 1 mg/ml cefotaxime for 30 h. The nuclear and cytoplasmic extracts were subjected to immunoblotting with the indicated antibodies

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