Fig. 1: Climacostol impairs autophagy in in vivo melanoma.
From: Dysfunctional autophagy induced by the pro-apoptotic natural compound climacostol in tumour cells
Subcutaneous B16-F10 melanoma allografts were excised from mice at day 16 of treatment (from day 0 - every 3–4 days) with 100 µl climacostol (CLIMA; 600 μg/ml) or control vehicle (CTRL). a, c Immunofluorescence imaging of LC3 and p62. DAPI was used for nuclei detection. Scale bar: 50 μm. Inserts represent enlarged image details. Lower panels: quantitative analysis of LC3 and p62 immunofluorescence. A total of 6 different images were analysed per tumour. Results are expressed as fold change of CTRL. b, d Western blotting images of LC3 and p62 expression. LDH was used as internal standard. Lower panels: densitometric analysis of LC3-II and p62 relative to their respective standard. Results are expressed as fold change of CTRL. Images and data represent the results obtained from 6 animals per experimental group. **p < 0.01 and ***p < 0.0001 relative to CTRL
