Fig. 5: Climacostol effects in multiple tumour cell lines.
From: Dysfunctional autophagy induced by the pro-apoptotic natural compound climacostol in tumour cells
MTT assay assessing the viability of A375 (a), SK-MEL-5 (b), GL261 (c), and U87MG (d) cells treated with increasing concentrations of climacostol for 24 h. Data are expressed by setting the absorbance of the reduced MTT in the absence of climacostol as 100%. The data points are representative of 8 independent experiments. Western blotting images of cleaved-caspase 3, LC3 and p62 expression in A375 (e), SK-MEL-5 (f), GL261 (g), and U87MG (h) cells cultured in the presence of 30 μg/ml climacostol (CLIMA) or control vehicle (CTRL) for 24. LDH was used as internal standard. Images are representive of 3 independent experiments
