Fig. 5: Overexpression of GIT1 promoted autophagic flux in HEK293T cells in vitro.

a, b The control and GIT1-overexpressing HEK293T cells were transfected with mRFP–GFP–LC3. Effect of GIT1 overexpression in promoting LC3 puncta formation under non-starvation and starvation conditions. Shown are LC3 fluorescent signals from representative single cells. Representative images (a) and data summary (b) are shown (**p < 0.01, Kruskal–Wallis test). Scale bar = 20 μm