Fig. 5: CUL1 knockdown inhibited CXCL8 and IL11 expressions to suppress migration and angiogenesis. | Cell Death & Disease

Fig. 5: CUL1 knockdown inhibited CXCL8 and IL11 expressions to suppress migration and angiogenesis.

From: CUL1 promotes breast cancer metastasis through regulating EZH2-induced the autocrine expression of the cytokines CXCL8 and IL11

Fig. 5

a Microarray analysis showed the fold changes of CXCL8 and IL11 in the CUL1 knockdown group compared with control groups. b Real-time PCR was used to validate the mRNA expressions of CXCL8 and IL11 in CUL1 knockdown and vector control MDA-MB-231 cells. c MDA-MB-231 cells were transiently transfected with CUL1 siRNA (si-CUL1) and vector siRNA control (si-Ctrl) for 48 h, and the serum-free medium was added to the cells for 24 h, then the conditioned medium was collected. ELISA analysis was used to test the secreted CXCL8 and IL11 expression levels in the 24 h conditioned medium. d The correlation of CUL1 mRNA expressions with CXCL8 and IL11 mRNA expressions in TCGA breast cancer dataset. CUL1 expression was divided into high and low groups based on the average value. e MDA-MB-231 cells were seeded in serum-free medium in the upper chamber and the 24 h conditioned medium from CUL1 knockdown and vector control MDA-MB-231 cells containing either supplementary recombinant CXCL8 and IL11 proteins or nothing was placed in the lower chamber, then the representative migration images of MDA-MB-231 cells were showed. f The number of cell migration per field was counted in five random fields (n = 3/group). g The tube formation by HUVECs in the conditioned medium from MDA-MB-231 cells with CUL1 knockdown and vector control which was complemented recombinant CXCL8 and IL11 proteins. f The number of tubes formed per field was counted in five random fields (n = 3/group) *P < 0.05, **P < 0.001 (Student’s t-test)

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