Fig. 6: EZH2 was an important transcriptional factor in CUL1 promoting cytokine gene expressions. | Cell Death & Disease

Fig. 6: EZH2 was an important transcriptional factor in CUL1 promoting cytokine gene expressions.

From: CUL1 promotes breast cancer metastasis through regulating EZH2-induced the autocrine expression of the cytokines CXCL8 and IL11

Fig. 6

a The activation Z-score arithmetic was used to predict the upstream transcriptional regulators of CXCL8 and IL11. b Western blotting was used to test the protein expression levels of predicted common upstream regulators ZEH2 and NFKBIA. c The correlation of CUL1 mRNA expressions with EZH2 mRNA expressions in 1104 TCGA breast invasive carcinomas from starBase v3.0 project. d Western blotting was used to test the expression of CUL1 and EZH2 in the MDA-MB-231 cells transfected with pCMV-EZH2 or vector plasmid, together with either si-CUL1 or si-Ctrl. e Real-time PCR was used to explore the CXCL8 and IL11 expressions in the MDA-MB-231 cells transfected with pCMV-EZH2 or vector plasmid, together with either si-CUL1 or si-Ctrl. f MDA-MB-231 cells were transiently transfected with pCMV-EZH2 or PCMV vector plasmid, together with either si-CUL1 or si-Ctrl for 48 h, and the serum-free medium was added to the cells for 24 h, then the conditioned medium was collected. ELISA analysis was used to test the protein expression levels of CXCL8 and IL11 in the 24 h conditioned medium. *P < 0.05, **P < 0.001 (Student’s t-test)

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