Fig. 5: The effect of SIRT2 on angiogenesis depends on the STAT3/VEGFA signalling pathway in CRC. | Cell Death & Disease

Fig. 5: The effect of SIRT2 on angiogenesis depends on the STAT3/VEGFA signalling pathway in CRC.

From: Inhibition of SIRT2 limits tumour angiogenesis via inactivation of the STAT3/VEGFA signalling pathway

Fig. 5

a VEGFA mRNA levels were examined by using qPCR assay for the indicated cells (ShNC SW480 cells, ShSIRT2 SW480 cells and ShSIRT2 SW480 cells transfected with STAT3C). Each bar represents the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01. b VEGFA production was measured in the culture supernatants. Each bar represents the mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001. c, d The migratory ability of HUVECs was assessed by using a transwell assay in the indicated cells. The number of migrated cells is shown in the bar graph. Each bar represents the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01. e, f The tube formation of HUVECs treated with different CM was observed, and the number of tubes formed is shown in the bar graph. Each bar represents the mean ± SD of three independent experiments. **P < 0.01

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