Table 1 Primer sequence
Gene | Sequence |
|---|---|
APC for qRT-PCR | F: 5′-AAAATGTCCCTCCGTTCTTATGG-3′ |
R: 5′-CTGAAGTTGAGCGTAATACCAGT-3′ | |
Axin2 for qRT-PCR | F: 5′-AGCCAAAGCGATCTACAAAAGG-3′ |
R:5′-AAGTCAAAAACATCTGGTAGGCA-3′ | |
GSK-3β for qRT-PCR | F: 5′-GGCAGCATGAAAGTTAGCAGA-3′ |
R: 5′-GGCGACCAGTTCTCCTGAATC-3′ | |
GAPDH for qRT-PCR | F: 5′-TGCACCACCAACTGCTTAGC-3′ |
R: 5′-GGCATGGACTGTGGTCATGAG-3′ | |
GSK-3β promoter for PCR | F: 5′-GGGGTACCTGTTAAATATCCGT GCCGATCT-3′ |
R: 5′-CCAAGCTTAGGAGGTCTAATAA TTTCAGATCCT-3′ | |
Axin2 promoter for PCR | F: 5′-GGGGTACCGCTGGAAACAGGACC TGCGT-3′ R: 5′- CCAAGCTTGACAGGCATGGGTTT GGTGA-3′ |
Axin2 for CHIP-qPCR | 5′-GGAGCAGTAAAAGGCCGTAA-3′ |
5′-CCAAACCATTGAAGCCCTTA -3′ | |
GSK-3β for CHIP-qPCR | 5′-CAGAGACGCTGGTGAAACTG-3′ |
5′-CCCCTTCTCTTCACCAATCA-3′ |
