Fig. 4: The effect of TNFR1 and TNFR2 on iTreg stability and function in vivo.

iTreg were induced from three strains (Thy1.2⁺) as above protocols. 3 days later, these cells (0.6 × 10⁶) were i.v. injected into Rag1^−/− mice that has received WT Thy1.1⁺ naive CD4⁺ T cells (0.6 × 10⁶) through i.p. injection on the same day. a Weights of the recipient mice were monitored after the cell transfer. b 56 days after the transfer, spleens, mLNs, and cLP were harvested and the proportions of Foxp3⁺ T cells gated on Thy1.2⁺ cells were determined. c The proportions of IL-17A⁺ or IFN-γ⁺ T cells gated on Thy1.1⁺ cells in cLP were determined. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001, error bars denote SD. Representative data is from six similar independent experiments