Fig. 3: EPO promotes Mcl-1 protein expression in DNR-treated cells. | Cell Death & Disease

Fig. 3: EPO promotes Mcl-1 protein expression in DNR-treated cells.

From: Erythropoietin inhibits chemotherapy-induced cell death and promotes a senescence-like state in leukemia cells

Fig. 3

a DA3/EPOR cells were cultured in the absence of EPO for 6 h followed by 6 h of treatment with DNR (0.25 µM) in the presence or absence of EPO (1 U/ml). Mcl-1 protein expression was determined by western blot analysis. β-actin levels serve as a loading control. The blot shown is representative of two independent experiments. b RT-qPCR analysis of Mcl-1 RNA. RNA was prepared from cells treated as in (a). Ct values were normalized to 18S rRNA. Error bars represent SEM (N = 3 biological replicates). One-way ANOVA did not return a significant f-statistic. Control cells were cultured in complete medium and were not treated with DNR

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