Fig. 5: EPO increases Mdm2 expression after DNR or nutlin-3a treatment and suppresses nutlin-3a-induced apoptosis. | Cell Death & Disease

Fig. 5: EPO increases Mdm2 expression after DNR or nutlin-3a treatment and suppresses nutlin-3a-induced apoptosis.

From: Erythropoietin inhibits chemotherapy-induced cell death and promotes a senescence-like state in leukemia cells

Fig. 5

Western blot showing p53 and Mdm2 protein levels in cells treated with DNR (a, b) or with nutlin-3a (c, d) in the presence or absence of EPO. In (a) and (b), DA3/EPOR cells were cultured in the absence of EPO for 1 h followed by 10 h of treatment with DNR (0.25 µM) with or without EPO (1 U/ml). In (c) and (d), cells were cultured in the absence of EPO for 0.5 h followed by 24 h of treatment with nutlin-3a (10 µM) with or without EPO (1 U/ml). Protein expression levels were determined by western blot analysis using the indicated antibodies. The HDM2-323 antibody recognizes full-length MDM2 and the SMP14 antibody recognizes the MDM2 p60 cleavage product. (e) Cells were treated as in (c, d) and the proportion of cells undergoing apoptosis (cells having <2 N DNA content) was measured by flow cytometry after staining with propidium iodide

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