Fig. 2: HACE1 expression in high vs low metastatic osteosarcoma cells.

a Immunoblot showing HACE1 protein expression in clonally derived pairs of high/low metastatic osteosarcoma cells, MG63.3/MG63 and MNNG/HOS. GAPDH was used as a loading control. b Immunofluorescence analysis of HACE1 expression in pairs of high/low metastatic MG63.3/MG63 and MNNG/HOS osteosarcoma cell line pairs. Blue: DAPI (nuclear stain); red: HACE1. Scale bars, 10 μm. c Quantitation of HACE1 signal intensity in b. Error bars indicate SEM for n = 10 HPFs. *p-value < 0.05, **p-value < 0.005