Fig. 1: ISL1 expression in primary GC tissues.

a–c The expression of ISL1 in primary GC (T) and corresponding surgical margin (N) tissues was examined by RT-PCR (a), western blotting (b), and immunohistochemical staining (c). Original magnification: 200× in c. d Association between ISL1 expression status and TNM stage. e, f Kaplan–Meier survival curves of disease-free survival (DFS) and overall survival (OS) for patients with ISL1-negative vs. ISL1-positive staining in GC tissues