Fig. 3: ISL1 overexpression promoted cell growth, migration, and invasion in MKN28 cells.

a Cell proliferation was monitored with an IncuCyte system every 6 h. b Plate colony formation assay. c Soft agar colony formation assay. d Assessment of invasion of MKN28 cells using Matrigel or Boyden chambers. e Wound healing assay by live-cell imaging on an IncuCyte system. Each bar in the bar charts of a–e represents the mean ± SD from three independent experiments of six replicate wells. *P < 0.05, **P < 0.01