Fig. 2: Thymidine phosphorylase (TP) stimulates the pentose Warburg effect on hepatocellular carcinoma (HCC) cells. | Cell Death & Disease

Fig. 2: Thymidine phosphorylase (TP) stimulates the pentose Warburg effect on hepatocellular carcinoma (HCC) cells.

From: Thymidine phosphorylase promotes malignant progression in hepatocellular carcinoma through pentose Warburg effect

Fig. 2

a Overexpression of Twist1 activated a series of pathways related to glycometabolism and amino acid metabolism. b Enrichment analysis of the gene expression profiles in PLC-PRF-5 cells overexpressing Twist1 detected by gene set enrichment analysis. c Enzyme-linked immunosorbent assay was used to analyze the TP factor levels in the medium. Overexpression of TP promoted the amount of TP secreted in PLC-PRF-5 cells; knocking down of TP reduced the amount of TP secreted in Hep3B cells. d Correlation between Twist1/TP expression levels and secreted TP factor levels in PLC-PRF-5 and Hep3B cells. e Overexpression of TP promoted the enzymatic metabolism of extracellular dT in the medium without glucose. f Enzymatic metabolism of extracellular dT regulated by TP promoted HCC cell proliferation. g Enzymatic metabolism of extracellular dT regulated by TP promoted ATP generation in PLC-PRF-5 cells. h Enzymatic metabolism of extracellular dT regulated by TP affected glycometabolism and amino acid metabolism in glucose-free cultured PLC-PRF-5 cells. NG means “No Glucose.” (mean ± SD; n = 3 in triplicate; *P < 0.05; **P < 0.01)

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