Fig. 4: Tetrandrine and DIM enhanced the degradation of Syk though the non-genomic route of AhR. RAW264.7 cells were transfected with either siArnt or control siCtrl, and then treated with tetrandrine or DIM for 6 h. | Cell Death & Disease

Fig. 4: Tetrandrine and DIM enhanced the degradation of Syk though the non-genomic route of AhR. RAW264.7 cells were transfected with either siArnt or control siCtrl, and then treated with tetrandrine or DIM for 6 h.

From: Tetrandrine enhances the ubiquitination and degradation of Syk through an AhR-c-src-c-Cbl pathway and consequently inhibits osteoclastogenesis and bone destruction in arthritis

Fig. 4

a CYP1A1 protein expression was detected using western blots assay. b The cells were treated with CHX (20 μg/mL) for 2 h, and exposed to RANKL (100 ng/mL) for 2 h. The cell lysates were analyzed using western blots with antibodies against Syk or GAPDH. Results are expressed as the means ± S.E.M. from at least 3 independent experiments. *P < 0.05, **P < 0.01 vs. indicated group. siAhR: AhR siRNA. siCtrl: control siRNA

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