Fig. 6: Tetrandrine and DIM enhanced the activation of c-Cbl through the AhR-c-src pathway.

a RAW264.7 cells were treated with or without tetrandrine (0.3 μM) for 6 h, and then treated with RANKL (100 ng/mL) for indicated periods. The levels of p-c-src and c-src were evaluated by western blots. b RAW264.7 cells were treated with tetrandrine (0.3 μM) or DIM (10 μM) for 6 h, and followed with RANKL (100 ng/mL) for 15 min. The levels of p-c-src and c-src were evaluated by western blots. c, d RAW264.7 cells were treated with tetrandrine (0.3 μM) or DIM (10 μM) for 6 h in the presence or absence of CH223191 (3 μM) or siAhR, and followed with RANKL (100 ng/mL) for 15 min. The levels of p-c-src and c-src were evaluated by western blots. e RAW264.7 cells were treated with tetrandrine (0.3 μM) or DIM (10 μM) for 6 h in the presence or absence PP2 (5 μM), and followed with RANKL (100 ng/mL) for 15 min. The levels of p-c-Cbl and c-Cbl were evaluated by western blots. f RAW264.7 cells were transfected with either sic-Cbl or siCtrl, and followed by treatment with tetrandrine (0.3 μM) or DIM (10 μM) for 6 h, and then exposed to RANKL (100 ng/mL) for 15 min. The levels of p-c-Cbl and c-Cbl were evaluated by western blots. Results are expressed as the means ± S.E.M. from at least 3 independent experiments. *P < 0.05, **P < 0.01 vs. indicated group. siAhR: AhR siRNA. siCtrl: control siRNA. sic-Cbl: c-Cbl siRNA