Fig. 2: Promotion of the induction of tumor-associated (i)Tregs by S1P1 in vitro. | Cell Death & Disease

Fig. 2: Promotion of the induction of tumor-associated (i)Tregs by S1P1 in vitro.

From: Sphingosine 1 phosphate receptor-1 (S1P1) promotes tumor-associated regulatory T cell expansion: leading to poor survival in bladder cancer

Fig. 2

a–c CD4+ cells and BC-derived cells were cocultured, after the BC-derived cells were treated with lenti-shS1P1-vector or with anti-TGF-β or anti-IL-10 antibodies (a), lenti-S1P1-expressing vector and S1P1 agonist (FTY720) (b), or lenti-S1P1-expressing vector and either anti-TGF-β or anti-IL-10 antibody (c) for 48 h. The percentage of CD4+Foxp3+Tregs were measured by FACS. d A statistical analysis showed the proliferation of OKT3-stimulated PBMCs, including CD4 and CD8 cells, after coculture with (i)Treg cells induced from BC-derived cells that were treated with lenti-shS1P1-vector, lenti-S1P1-expressing vector, lenti-control vector or lenti-S1P1-expressing vector combined with either TGF-β or IL-10 antibody for 5 days. The data shown were obtained from one of three experiments, and the statistical analyses were performed using Student’s t-test. The bars represent the SEMs from three experiments. *P < 0.05. **P < 0.01

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