Fig. 5: MTORC1 inhibition correlates with ULK-1 activation and large acidic vacuoles. | Cell Death & Disease

Fig. 5: MTORC1 inhibition correlates with ULK-1 activation and large acidic vacuoles.

From: CK2 inhibition with silmitasertib promotes methuosis-like cell death associated to catastrophic massive vacuolization of colorectal cancer cells

Fig. 5

a Colon cancer (SW-480, DLD-1, HT-29, and HCT-116) and non-tumor (CoN) cells were treated with 25 μM silmitasertib for 6 h and photographed under a phase-contrast microscope. b DLD-1 cells were treated with silmitasertib at increasing concentrations (12.5, 25, and 50 μM) for different times (3, 6, 12, 24, and 48 h) and photographed under a phase-contrast microscope. Images taken at 20x were processed for quantification of number (c) and size (d) of vacuoles per single cell as in Materials and methods. e DLD-1 cells were transfected with either a specific siRNA for CK2α or a scrambled siRNA (control) by using Lipofectamine 2000 at a 1:3 ratio. Phase-contrast images were obtained at 0, 6, and 24 h post-transfection and quantification of size (gray bars) and number of vacuoles per single cell (black bars) were determined. Data represents the average ± SEM (n = 3); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns: not significant. Bar: 20 μm

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