Fig. 6: Silmitasertib-derived vacuoles are acidic but negative for LC3-II and p62. | Cell Death & Disease

Fig. 6: Silmitasertib-derived vacuoles are acidic but negative for LC3-II and p62.

From: CK2 inhibition with silmitasertib promotes methuosis-like cell death associated to catastrophic massive vacuolization of colorectal cancer cells

Fig. 6

a DLD-1 cells treated with 25 μM silmitasertib for 6 h were then labeled with 1 μM lysotracker (green) for 30 min and living cells were visualized by video-microscopy. Black arrowheads indicate the vacuoles highlighted in white squares. Representative images are shown (n = 3), scale bars: 10 μm. b DLD-1 cells were transfected with a plasmid encoding GFP-LC3. After a 24 h transfection, cells were treated with 25 μM silmitasertib for 6 or 12 h and visualized by confocal microscopy. White arrowheads indicate LC3 puncta. c DLD-1 cells were treated during 6 h with 25 μM silmitasertib and/or 100 nM bafilomycin-A1 (BAF), followed by analysis of LC3-I/II and p62 levels by western blot. d DLD-1 cells were treated with 25 μM silmitasertib and the last 3 h with 100 nM BAF, then cultured for another 3, 6, 12, and 18 h, followed by analysis of LC3-I/II levels by western blot

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