Fig. 5: PD-NSCs showed autophagy dysfunction during the aging process after IR. | Cell Death & Disease

Fig. 5: PD-NSCs showed autophagy dysfunction during the aging process after IR.

From: Stress-induced precocious aging in PD-patient iPSC-derived NSCs may underlie the pathophysiology of Parkinson’s disease

Fig. 5

a BECN1 expression in PD-NSCs after irradiation treatment was significantly decreased as revealed by western blot and densitometric analysis. b The mRNA expression of several genes linked with autophagy function, such as BECN1, LC3B, ATG5, ATG7, and ATG12, was consistently decreased in PD-NSCs with IR treatment. c The expression of LC3B in PD-NSCs with irradiation was further reduced as detected by immunostaing. Scale bar: 10 μm. d, e The double membrane structures of autophagy vacuoles in PD-NSCs after oxidative stress were decreased as analyzed by electron microscope under a JEM 1010 transmission electron microscope. NSCs were treated with H2O2 and incubated for 48 h, and then were fixed and stained. The yellow asterisk indicated autophagic vacuoles, including autophagosomes. Scale bar: 0.5 μm. f, g Distribution of GFP-LC3 in PD-NSCs changed dramatically under post-H2O2 conditions. After cells were treated with H2O2 for 48 h, they were infected with RFP-GFP-LC3 virus. Scale bar: 50 μm. All the data were expressed as mean ± SD from three independent experiments. Statistical analysis was performed by Student’s t-test, *P < 0.05; **P < 0.01; ***P < 0.001; ns not statistically significant. All data were obtained from at least three independent experiments

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