Fig. 7: SIRT1 could regulate autophagy in the aging process of PD-NSCs.

a Reduction of BECN1 protein levels in PD-NSCs by IR treatment was restored by 3 μM resveratrol treatment, analyzed by western blotting and densitometry. b The decrease of mRNA expression of autophagy-related genes, such as BECN1, LC3B, ATG5, ATG7, and ATG12 in PD-NSCs treated with IR was prevented by administration of 3 μM resveratrol. c The suppression of LC3B expression in PD-NSCs after IR treatment was restored in the presence of 3 μM resveratrol. d The expression of BECN1 in WT-NSCs with Sirt1 knockdown decreased significantly compared with control group, analyzed by western blotting and densitometry. e The mRNA expression of autophagy-related genes, such as BECN1, LC3B, ATG5, ATG7, and ATG12 in WT-NSCs treated with IR was further reduced when Sirt1 expression was inhibited. f The expression of LC3B decreased by knockdown of Sirt1 in WT-NSCs after IR treatment. g The decline of BECN1 expression in PD-NSCs after IR was inhibited by overexpression of Sirt1, analyzed by western blotting and densitometry. h The reduction of LC3B in PD-NSCs post IR was alleviated after Sirt1 was upregulated. The data were expressed as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ns not statistically significant, Student’s t-test. All data were obtained from at least three independent experiments. Scale bar = 100 μm