Fig. 1: Deep sequencing of small RNAs associated with gemcitabine response.

a, b Drug-resistance cell line was established as described in the Materials and methods. Gemcitabine sensitivity of both SW1990-R cells and its parental cells (a) were tested by MTS assay and the IC50 values were calculated (b). *P < 0.05 and **P < 0.01, compared with control conditions. c Sequence length distribution in SW1990-R and SW1990 cells. d Summary of sequencing reads mapped to various types of sRNA in SW1990-R and SW1990 cells. e Cluster analysis of changed miRNAs in SW1990-R cells compared with SW1990 cells. A line indicates a microRNA gene, and a column indicates a sample. Two samples were contained in each group. Both upregulated (red) and downregulated miRNAs (blue) were identified. f Expressions of ten identified miRNAs were detected by qRT-PCR to validate microarray data. *P < 0.05, compared with SW1990. Points, mean values for three independent experiments; error bars, ±SEM