Fig. 3: MiR-30a suppresses cell proliferation and enhances cell sensitivity to gemcitabine.

a, b SW1990 cells were transfected with miR-30a precursor (miR-30a) or scramble control lentivirus (miR-Con). MiR-30a level was confirmed by qRT-PCR (a). Gemcitabine sensitivity was determined by MTS assays (b). *P < 0.05 and **P < 0.01, compared with miR-Con. c, d SW1990 cells were transfected with miR-30a inhibitor or scramble control (miR-Con). MiR-30a expression was detected by qRT-PCR (c). Gemcitabine sensitivity was determined by MTS assays (d). *P < 0.05 and **P < 0.01, compared with miR-Con. e SW1990 cells were transfected with indicated constructs and then treated with 2 μM gemcitabine for 72 h. The colony forming assay was performed. Representative micrographs (left) and quantification (right) of crystal violet-stained cell colonies were displayed. f SW1990 cells were transfected with indicated constructs. Cells were then treated and detected as in e. g, h SW1990 cells were transfected with miR-30a or miR-Con. Cell growth were determined by MTS assays (g). Representative micrographs (left) of colony formation, as well as quantification (right) of crystal violet-stained cell colonies were displayed (h). n = 3 wells per group. *P < 0.05 and **P < 0.01, compared with miR-Con. i, j SW1990 cells were transfected with miR-30a inhibitor or miR-Con. Cell growth and colony formation assay were determined as in g and h, respectively. n = 3 wells per group. *P < 0.05 and **P < 0.01, compared with miR-Con. k, l SW1990-R cells were transfected with miR-30a precursor (miR-30a) or scramble control lentivirus (miR-Con). MiR-30a level was confirmed by qRT-PCR (k). Gemcitabine sensitivity was determined by MTS assays (l). *P < 0.05 and **P < 0.01, compared with miR-Con. m SW1990-R cells after transfection with indicated constructs were treated with 6uM gemcitabine for 72 h. The colony forming assay was performed as in e. Points, mean values for three independent experiments; error bars, ±SEM