Fig. 2: The unfolded protein response (UPR) is an early event during in vivo hepatic stellate cell (HSC) activation.
From: Unfolded protein response is an early, non-critical event during hepatic stellate cell activation
a BALB/c mice received a single CCl4 injection. After the indicated time points, i.e., 2, 6, 10 and 24 h, HSCs were isolated and immediately collected for mRNA analysis of activation and endoplasmic reticulum (ER) stress markers. The graphics show the results of two different HSC isolations (each time point = 3 mice). b Immunofluorescent staining for Bip (green) of HSCs which were isolated, cytospinned and fixed at different time points after a single CCl4 injection. 4′,6-Diamidino-2-phenylindole (DAPI; blue) was used as a nuclear staining. Graph representing the quantification of % mean intensity in at least 25 cells for each time point, from 3 independent isolations. c mRNA analysis of ER stress markers in HSCs isolated after multiple CCl4 injections (harvested 24 h after the last injection); n = 5. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 versus control
