Fig. 4: ASS1P3 could regulate ASS1 expression via miR-34a-5p. | Cell Death & Disease

Fig. 4: ASS1P3 could regulate ASS1 expression via miR-34a-5p.

From: Androgen receptor regulates ASS1P3/miR-34a-5p/ASS1 signaling to promote renal cell carcinoma cell growth

Fig. 4

a Real-time RT-PCR assay was applied to detect the expression of ASS1 in Caki-1 cells with overexpressed ASS1P3 and in SW-839 cells with knocked-down ASS1P3. b Argonaute2 immunoprecipitation assay was performed after transfecting A498 and OSRC-2 cells with functional AR-cDNA or vector. c Argonaute2 immunoprecipitation assay was performed after transfecting SW-839 and OSRC-2 cells with AR-shRNA or vector. d Cartoon of screening for miRNA candidates. e Western blot assay was applied to detect the expression of ASS1 in OSRC-2 and SW-839 cells after overexpressing miR-34a-5p. f Western blot assay showed that oemiR-34a-5p could partially reverse the AR-shRNA-increased ASS1 expression in OSRC-2 and SW-839 cells. For (a–c), data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ns = not significant, compared to the controls

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