Fig. 5: ASS1P3 functions as a miRNA sponge for miR-34a-5p to regulate ASS1 in RCC. | Cell Death & Disease

Fig. 5: ASS1P3 functions as a miRNA sponge for miR-34a-5p to regulate ASS1 in RCC.

From: Androgen receptor regulates ASS1P3/miR-34a-5p/ASS1 signaling to promote renal cell carcinoma cell growth

Fig. 5

a Sequence alignment of the ASS1 3′UTR with wild type versus potential mutant miR-34a-5p targeting sites. b, c Co-transfection of wild type or mutant seed regions of ASS1 3′UTR constructs with miR-34a-5p in SW-839 (b) and OSRC-2 (c) cells. The luciferase assay was applied to detect the luciferase activity. d Pull-down assay was performed by biotinylated antisense oligo specific for ASS1P3 and ASS1 after transfecting cells with functional AR-cDNA, AR-shRNA, or vector control in OSRC-2 and SW-839 cells. e, f Co-transfection of wild type or mutant seed regions of ASS1 3′UTR constructs with miR-34a-5p, ASS1P3, pLVTHM, and pWPI in SW-839 (e) and OSRC-2 (f) cells for luciferase assay to detect the luciferase activity. g Overall survival of RCC patients was analyzed according to miR-34a-5p expression based on the TCGA dataset. h Correlation analysis of miR-34a-5p and ASS1 mRNA levels by Pearson correlation coefficient analysis in the TCGA dataset. For b–f, data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ns = not significant, compared to the controls

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