Fig. 3: ZFP57 suppresses the Wnt/β-catenin pathway in breast cancer cells.

a Expression of β-catenin, cyclin D1, c-Myc in the ZFP57-overexpressed or -repressed cells as compared with their controls was detected by qRT-PCR. Data are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001. b Expression of β-catenin, cyclin D1, c-Myc in the ZFP57-overexpressed or -repressed cells as compared with their controls was detected by Western blot. c Schematic diagram of TOPFlash and mutated FOPFlash. d The effect of ZFP57 on the Wnt/β-catenin signalling pathway was assessed by dual-luciferase reporter assays (TOPFlash and FOPFlash) in the ZFP57-overexpressed or -repressed cells as compared with their controls. Data are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001