Fig. 5: Methylation levels of MEST promoter region in breast cancer tissues and cells, which could be regulated by ZFP57. | Cell Death & Disease

Fig. 5: Methylation levels of MEST promoter region in breast cancer tissues and cells, which could be regulated by ZFP57.

From: ZFP57 suppress proliferation of breast cancer cells through down-regulation of MEST-mediated Wnt/β-catenin signalling pathway

Fig. 5

a Representative MS-PCR analysis detected methylation levels of MEST promoter region in ten pairs of human breast cancer tissues and adjacent normal tissues. 1–10, Tissue sample NO.; T, tumour tissue; N, normal tissue; M, methylation; U, unmethylation. b Representative MS-PCR analysis detected methylation levels of MEST promoter region in breast cancer (MCF-7 and SUM1315) cells. c–e Representative MS-PCR analysis (c) of MEST, qRT-PCR (d) and Western blot (e) of MEST expression MCF-7 with or without 5-AzaDc treatment. Data are presented as mean ± SD. **p < 0.01. f Representative MS-PCR analysis of MEST in the ZFP57-overexpressed or -repressed cells as compared with their controls

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