Fig. 1: IFNβ induced the expression of soluble mediators and promoted MSC immunomodulatory function. | Cell Death & Disease

Fig. 1: IFNβ induced the expression of soluble mediators and promoted MSC immunomodulatory function.

From: IFNβ enhances mesenchymal stromal (Stem) cells immunomodulatory function through STAT1-3 activation and mTOR-associated promotion of glucose metabolism

Fig. 1

a Real-time PCR analysis of immunoregulatory genes in MSC exposed or not to IFNβ. Data are mean ± SD (n = 3); b Immunofluorescence analysis of Slpi expression in MSC. Red, SLPI; blue, DAPI; c Real-time PCR analysis of Slpi expression was evaluated in MSC following transfection with specific siRNA (MSC SLPI-KD) and compared to that of control MSC (MSC CTRL-KD). Data are mean ± SD (n = 3); d In vitro T-cell proliferation assay in the presence of MSC CTRL-KD and MSC SLPI-KD. MSC were added at the ratio 1:40 with activated splenocytes. Data are presented as mean ± SD (n = 3); e In vitro T-cell proliferation in the presence of MSC conditioned medium (MSC CM) collected after priming with IFNβ (IFN CM). The CM of 3T3 fibroblast culture was used as control. Data are mean ± SD (n = 3)

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