Fig. 6: SIRT3 deacetylates ECHA and increases fatty acid β-oxidation in β cells. | Cell Death & Disease

Fig. 6: SIRT3 deacetylates ECHA and increases fatty acid β-oxidation in β cells.

From: The pivotal role of protein acetylation in linking glucose and fatty acid metabolism to β-cell function

Fig. 6

a Acetylation level of endogenous ECHA in INS-1 cells treated with or without 5 mM NAM for 6 h. b OCR of palmitate was measured after rat islets were treated with or without 5 mM NAM for 6 h. c ECHA protein level in INS-1 cells treated with or without 5 mM NAM for 6 h. d ECHA protein level in INS-1 cells treated with cycloheximide (CHX) for the indicated time. e After INS-1 cells were treated with or without NAM in the absence or presence of MG132, ECHA protein level was detected. f After rat islets were transfected with control vector (Ad-Vector) or SIRT3-overexpressing adenovirus (Ad-SIRT3), protein levels of SIRT3, SIRT4, and SIRT5 were detected. g After INS-1 cells were transfected with control vector or SIRT3-overexpressing adenovirus, cell lysates were immunoprecipitated with ECHA antibody and subjected to Western blot with anti-acetyllysine antibody. h INS-1 cells transfected with vector or SIRT3-overexpressing adenovirus were treated with cycloheximide for the indicated time and then ECHA protein level was detected. i Palmitate oxidation rate was measured after rat islets were transfected with control vector or SIRT3-overexpressing adenovirus. Data are expressed as mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, **p < 0.001 vs. control. #p < 0.05 vs. NAM

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