Fig. 5: Autophagy was alleviated by silencing p8 in cells treated with fisetin. | Cell Death & Disease

Fig. 5: Autophagy was alleviated by silencing p8 in cells treated with fisetin.

From: Fisetin induces autophagy in pancreatic cancer cells via endoplasmic reticulum stress- and mitochondrial stress-dependent pathways

Fig. 5

a Western blot detection of the expression of p53, PKCɑ, both of which were involved in p8-dependent pathway. b p8-specific siRNAs (100 pM) were transfected into PANC-1 cells with siRNA transfection reagent for 24 h. RT-qPCR of p8 in control (siNC) and p8 interference (p8si1, p8si2, and p8si3) PANC-1 cells with or without 200 μM fisetin treatment for 48 h was performed. The results are presented as the mean ± SD (n = 3), *P ≤ 0.05, **P ≤ 0.01,***P ≤ 0.001. c Western blotting analyses of p8 in control and p8 interference PANC-1 cells. d Western blotting analyses of PERK, ATF4, and ATF6 in control and p8 interference PANC-1 cells with or without fisetin treatment. e Western blotting analyses of LC3B in control and p8 interference PANC-1 cells with or without fisetin treatment. To promote autophagosome generation, cells were treated with 20 μM Chloroquine (CQ) for 2 h before collection. f Western blotting analyses of Parkin and PINK1 in control and p8 interference PANC-1 cells with or without fisetin treatment

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