Fig. 3: Preincubation with tumor cell supernatant promoted platelet aggregation and ATP release.
From: Cancer cell-derived immunoglobulin G activates platelets by binding to platelet FcγRIIa
Human washed platelets were pretreated with culture medium or cancer cell supernatant and CaCl2 (1 mM) for 15 min, and aggregation and ATP release were detected (a, b, e, f: 0.5 μg/mL collagen as agonist; c, d, g, h: 0.04 U/mL thrombin as agonist). Representative curves are shown. All experiments were repeated at least three times. Data are represented as the mean ± SEM. *, # P < 0.05, **, ## P < 0.01; and ***, ### P < 0.001 compared with the appropriate RPMI 1640 or DMEM medium group
