Fig. 4: Knockdown of Tor restores autophagy in the presence of Dpp.
From: Crosstalk between Dpp and Tor signaling coordinates autophagy-dependent midgut degradation
a Autophagy detected by Atg8a puncta in midgut cells from Dpp (NP1-GAL4/+; UAS-dpp/+) and TkvACT (NP1-GAL4/+; UAS-tkvACT/+) is greatly reduced compared to the control (NP1-GAL4/+) at −4 h RPF. Increased Atg8a puncta is observed in TorRNAi knockdown alone and when combined with Dpp (NP1-GAL4/+; UAS-dpp/UAS-TorRNAi) and TkvACT (NP1-GAL4/+; UAS-TorRNAi/UAS-TkvACT). Quantification of Atg8a puncta measured using ImageJ (data represent as the average puncta/cell ± SD; **p < 0.05). b LysoTracker Red shows low basal levels in Dpp and TkvACT midguts at −4 h RPF. Increased LysoTracker Red staining is observed in Tor knockdown alone and when combined with Dpp (NP1-GAL4/+; UAS-dpp/UAS-TorRNAi) and TkvACT (NP1-GAL4/+; UAS-TorRNAi/UAS-TkvACT). Quantification of LTR puncta measured using ImageJ (data represent as the average puncta/cell ± SD; *p < 0.05). Scale bar represents 100 μm (a, b)
