Fig. 1: Downregulation of cyclinB1 induced reactive oxygen species (ROS)-mediated autophagy. | Cell Death & Disease

Fig. 1: Downregulation of cyclinB1 induced reactive oxygen species (ROS)-mediated autophagy.

From: Downregulation of G2/mitotic-specific cyclinB1 triggers autophagy via AMPK-ULK1-dependent signal pathway in nasopharyngeal carcinoma cells

Fig. 1

a Three small interfering RNAs (siRNAs) were designed against the open reading frame of cyclinB1 mRNA, and silencing efficiency was detected by the western blot analysis. b Western blot for LC3B I, II, and p62 on treatment with non-coding siRNA (siNC) or cyclinB1 siRNA (siCB1) for 72 h. c Measurement of monodansylcadavarine-positive acidic vesicles, including autophagosomes, in CNE-1 and CNE-2 cells treated with siNC or siCB1 for 72 h by flow cytometry. Detection of d ATP and e cellular ROS and MitoSOX levels in both CNE-1 and CNE-2 cells upon transfection with siNC or siCB1 for 72 h. All data represented mean ± s.d. from three independent experiments; P values were calculated in comparison with cells treated with siNC (control) unless indicated. NS: P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

Back to article page