Fig. 2: Detection of autophagosome. | Cell Death & Disease

Fig. 2: Detection of autophagosome.

From: Downregulation of G2/mitotic-specific cyclinB1 triggers autophagy via AMPK-ULK1-dependent signal pathway in nasopharyngeal carcinoma cells

Fig. 2

a Representative transmission electron microscopic microphotograph of cells treated with siNC or siCB1 for 72 h. Black arrows indicated double-membrane autophagosome. Scale bars, 5 µm and 2 µm. Quantification of endogenous LC3 dots by b immunofluorescence and c RFP-GFP-LC3B puncta by RFP-GFP-LC3B dual reporter assay. All data represented mean ± s.d. from three independent experiments; P values were calculated in comparison with cells treated with siNC (control) unless indicated. NS: P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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