Fig. 2: CALCB expression is regulated through EWSR1-FLI1 binding to a nearby enhancer-like GGAA-microsatellite. | Cell Death & Disease

Fig. 2: CALCB expression is regulated through EWSR1-FLI1 binding to a nearby enhancer-like GGAA-microsatellite.

From: Targeting the CALCB/RAMP1 axis inhibits growth of Ewing sarcoma

Fig. 2

a Integrative genomic view of published ChIP-seq7 and DNAse-seq data30 of the CALCB locus. Data were generated in A673 and SK-N-MC Ewing sarcoma cells, stably transfected with either shRNA targeting GFP (shGFP; negative control) or EWSR1-FLI1 (shEF1). The blue box marks the location of the CALCB gene-associated GGAA-microsatellite, consisting of 16 repeats of the GGAA-motif in the human reference genome (hg19). b Luciferase reporter assays in A673/TR/shEF1 cells with/without knockdown of EWSR1-FLI1 (Dox+/−) 72 h after transfection with plasmids containing a 359-bp fragment around the CALCB-associated GGAA-microsatellite as displayed in a. Data are presented as mean (n = 3–5) and SEM; unpaired two-tailed Student’s t test. **P ≤ 0.01; ***P ≤ 0.001

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