Fig. 3: circTADA2A-E6 suppresses cancer progression.
From: circTADA2As suppress breast cancer progression and metastasis via targeting miR-203a-3p/SOCS3 axis
a–f Forty-eight hours after transfecting MCF-7 and MDA-MB-231 cells with a vector expressing circTADA2A-E6, ectopic circTADA2A-E6 expression was analyzed by quantitative reverse transcription polymerase reaction (qRT-PCR) (a). The effect of circTADA2A-E6 on cell viability was analyzed by CCK-8 (b). The effect of circTADA2A-E6 on colony formation after two- to three-week transfection was determined via a clonogenicity assay (left, representative pictures; right, quantitative bar for colony numbers) (c). The effect of circTADA2A-E6 on cell migration was determined via wound scratch assay (left, representative images; quantitative bar for cell migration rate) (d). The effect of circTADA2A-E6 on cell invasion was determined via Transwell assay (left, morphological comparison of cell penetration; quantitative bar for number of penetrating cells) (e). The effect of circTADA2A-E6 on the expression levels of E-cadherin and vimentin was analyzed by western blotting (WB) (f). Error bars represent the mean ± SEM from three independent experiments. *p < 0.05, **p < 0.01
