Fig. 4: AGAP2-AS1 regulates PC cell cycle arrest and apoptosis in vitro. | Cell Death & Disease

Fig. 4: AGAP2-AS1 regulates PC cell cycle arrest and apoptosis in vitro.

From: RREB1-induced upregulation of the lncRNA AGAP2-AS1 regulates the proliferation and migration of pancreatic cancer partly through suppressing ANKRD1 and ANGPTL4

Fig. 4

a–c Flow cytometry was used to determine and compare the differences in cell cycle progression when PC cells were transfected with si-NC and si-AGAP2-AS1. The bar graph represents percentage data for G0/G1, S, and G2/M phase cells. d, e Flow cytometry was used to determine and compare apoptosis differences in PC cell lines of the si-NC group and si-AGAP2-AS1 group. LL dead cells, UL viable cells, LR early apoptotic cells, UR terminal apoptosis cells. f, g A TUNEL staining assay was used to determine apoptosis of AsPc-1 and BxPC-3 cells transfected with si-NC and si-AGAP2-AS1. Green, apoptotic cells; blue, DAPI stained nuclei. h–j Protein levels of apoptosis-related proteins (PARP, cleaved PARP, Bak, and Bax) and G1–S-phase checkpoint proteins (such as cyclin D3 and CDK4) were detected by western blot analysis in AsPC-1 and BxPC-3 cells transfected with si-NC and si-AGAP2-AS1. *P < 0.05, **P < 0.01, ***P < 0.001

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