Fig. 8: Co-transfection partially reversed the apoptosis acceleration, proliferation inhibition, and migration inhibition induced by si-AGAP2-AS1. | Cell Death & Disease

Fig. 8: Co-transfection partially reversed the apoptosis acceleration, proliferation inhibition, and migration inhibition induced by si-AGAP2-AS1.

From: RREB1-induced upregulation of the lncRNA AGAP2-AS1 regulates the proliferation and migration of pancreatic cancer partly through suppressing ANKRD1 and ANGPTL4

Fig. 8

a, b EdU assays were performed to determine the cell viability for BxPC-3 cells transfected with si-NC, si-ANKRD1, si-AGAP2-AS1, si-AGAP2-AS1 + si-ANKRD1. c, d Flow cytometry assays were performed to analyze the cell apoptosis in for BxPC-3 cells transfected with si-NC, si-ANKRD1, si-AGAP2-AS1, and si-AGAP2-AS1 + si-ANKRD1. e, f Transwell assays were used to determine cell migration and invasion in AsPc-1 and BxPC-3 cells transfected with si-NC, si-ANGPTL4, si-AGAP2-AS1, and si-AGAP2-AS1 + si-ANGPTL4. g Bioinformatics prediction of the interaction probability of AGAP2-AS1 and RNA-binding proteins on a random forest (RF) or support vector machine (SVM) basis. h In the RIP experiments, co-precipitated RNA was detected by qRT-PCR. The fold enrichment of AGAP2-AS1 in the AsPc-1 and BxPC-3 cell was matched using IgG as a control. i Relative expression level of EZH2 in PC tissue (n = 46) compared with adjacent nontumor tissue (n = 46). Standardization was performed with reference to GAPDH expression. j MTT assays were performed to determine the cell viability for AsPc-1 and BxPC-3 cells transfected with si-EZH2. *P < 0.05, **P < 0.01, ***P < 0.001

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