Fig. 9: AGAP2-AS1 downregulates ANKRD1 and ANGPTL4 by interacting with EZH2. | Cell Death & Disease

Fig. 9: AGAP2-AS1 downregulates ANKRD1 and ANGPTL4 by interacting with EZH2.

From: RREB1-induced upregulation of the lncRNA AGAP2-AS1 regulates the proliferation and migration of pancreatic cancer partly through suppressing ANKRD1 and ANGPTL4

Fig. 9

a EdU assays were performed to determine the cell proliferation in AsPc-1 and BxPC-3 cells transfected with si-EZH2. b The relative expression levels of EZH2 in AsPc-1 and BxPC-3 cells transfected with si-NC or si-EZH2 were measured using qPCR. c The relative expression levels of ANKRD1 and ANGPTL4 in AsPc-1 and BxPC-3 cells transfected with si-NC or si-EZH2 were measured using qRT-PCR. d The cytoplasm and nuclei of AsPC-1 and BxPC-3 cells were isolated, and the levels of AGAP2-AS1 were detected by qRT-PCR with GAPDH as the cytoplasmic control, U1 as the nuclear control. The distribution of AGAP2-AS1 is expressed as a percentage of total RNA. e Confocal FISH images showing localization of AGAP2-AS1 in BxPC-3 cells. U6 was taken as representative of nuclear localization, and 18s as representative of cytoplasmic localization. Red, FISH probe; blue, DAPI nuclear staining. f ChIP results showing EZH2 occupancy on the ANKRD1 and ANGPTL4 promoter regions; knockdown of AGAP2-AS1 decreases their occupancy. IgG was used as a negative control. g Schematic diagram of the mechanism of AGAP2-AS1/EZH2/ANKRD1 and ANGPTL4 axis in PC cells. *P < 0.05, **P < 0.01, ***P < 0.001

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