Fig. 4: PI3K/AKT activates NF-κB/Snail axis to regulate NETO2-induced EMT. | Cell Death & Disease

Fig. 4: PI3K/AKT activates NF-κB/Snail axis to regulate NETO2-induced EMT.

From: NETO2 promotes invasion and metastasis of gastric cancer cells via activation of PI3K/Akt/NF-κB/Snail axis and predicts outcome of the patients

Fig. 4

a Western blotting analysis showed attenuated phosphorylation of p65, IKKβ, and IκBα in NETO2-knockdown cells compared to mock cells. b Western blotting analysis showed increased nuclear accumulation of NF-κB p65 in Over-NETO2 cells compared to control cells. c The NF-κB transcription activity was decreased in NETO2-knockdown gastric cancer cells, while increased in NETO2-overexpression gastric cancer cells. d Western blotting analysis showed that TNF-α (10 ng/mL) treatment resulted in increased phosphorylation and nuclear accumulation of p65, downregulated E-cadherin and upregulated N-cadherin in sh-NETO2–1 cells. e The mRNA level of Snail was upregulated after treatment with TNF-α (10 ng/mL) in sh-NETO2–1 GC cells. f Treatment with TNF-α (10 ng/mL) enhanced NF-κB transcription activity measured by dual luciferase assay in sh-NETO2–1 GC cells. g Quantification of transwell invasion assay showed increased invasive ability after treatment with TNF-α (10 ng/mL) in sh-NETO2–1 GC cells. h Western blotting analysis showed that JSH-23 (10 μM) treatment led to decreased phosphorylation and nuclear accumulation of p65, upregulated E-cadherin, and downregulated N-cadherin in Over-NETO2 cells. i The mRNA level of Snail was decreased after treatment with JSH-23 (10 μM) in Over-NETO2 GC cells. j Treatment with JSH-23 (10 μM) decreased the NF-κB transcription activity measured by dual luciferase assay in Over-NETO2 GC cells. k Quantification of transwell invasion assay showed decreased invasive ability after treatment with JSH-23 (10 μM) in Over-NETO2 GC cells. l Western blotting analysis showed that BAY 11–7082 (10 μM) treatment resulted in decreased phosphorylation and nuclear accumulation of p65, upregulated E-cadherin and downregulated N-cadherin in Over-NETO2 cells. m The mRNA level of Snail was decreased after treatment with BAY 11–7082 (10 μM) in Over-NETO2 GC cells. n Treatment with BAY 11–7082 (10 μM) decreased the NF-κB transcription activity measured by dual luciferase assay in Over-NETO2 GC cells. o Quantification of transwell invasion assay showed decreased invasive ability after treatment with BAY 11–7082 (10 μM) in Over-NETO2 GC cells. **p < 0.01, ***p < 0.001; NS no significant

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