Fig. 5: TNFRSF12A mediates NETO2-induced activation of PI3K/AKT/NF-κB/Snail axis. | Cell Death & Disease

Fig. 5: TNFRSF12A mediates NETO2-induced activation of PI3K/AKT/NF-κB/Snail axis.

From: NETO2 promotes invasion and metastasis of gastric cancer cells via activation of PI3K/Akt/NF-κB/Snail axis and predicts outcome of the patients

Fig. 5

a qRT-PCR analysis showed knockdown of NETO2 downregulated the mRNA level of TNFRSF12A, while overexpression of NETO2 upregulated the mRNA level of TNFRSF12A. b Western blotting analysis showed that depletion of NETO2 downregulated TNFRSF12A expression, while overexpression of NETO2 upregulated TNFRSF12A expression. c Depletion of TNFRSF12A in MGC803 and XN0422 cells inactivated PI3K/AKT/NF-κB pathway, but had no influence on Erk1/2 pathway. d Depletion of TNFRSF12A impaired the NF-κB transcription activity in MGC803 and XN0422 cells. e Silencing TNFRSF12A abrogated NETO2-induced activation of PI3K/AKT/NF-κB pathway. f Depletion of TNFRSF12A inhibited NETO2-induced NF-κB transcription activity; *p < 0.05, **p < 0.01, ***p < 0.001; NS no significant

Back to article page