Fig. 3: Transforming growth factor beta (TGFβ)/tumor necrosis factor alpha (TGFβ/TNFα) treatment regulates histone H3 modifications.
From: EMT is associated with an epigenetic signature of ECM remodeling genes
a Histones were purified from ACHN cells treated with or without TGFβ/TNFα for 5 days. Twenty-one posttranslational modifications of histone H3 (acetylation: H3K9ac, H3K14ac, H3K18ac, H3K56ac; methylation: H3K4me1, H3K4me2, H3K4me2, H3K9me1, H3K9me2, H3K9me3, H3K27me1, H3K27me2, H3K27me3, H3K36me1, H3K36me2, H3K36me3, H3K79me1, H3K79me2, H3K79me3; and phosphorylation: H3S10P, H3S28P) were quantified using a multiplex enzyme-linked immunosorbent assay. Values were normalized to total H3 content in the same measurement. Each bar represents the mean of two independent measurements and each one was obtained from a mix of two independent histone extractions. b, c Increase staining of H3K4me2, H3K9me3, and H3K27me3 marks observed using immunofluorescence in A549, ACHN, and MCF10A cells treated with or without TGFβ/TNFα for 5 days. Each picture is representative of a typical result from at least three independent experiments. d Increased staining in H3K4me2, H3K9me3, and H3K27me3 marks quantified using flow cytometry in A549, ACHN, and MCF10A cells treated with or without TGFβ/TNFα for 5 days (representative results of at least 3 independent experiments)
