Fig. 5: Sphingosine-1-phosphate receptor 1 (S1PR1) promoted VE-cadherin phosphorylation and the secretion of vascular endothelial growth factor (VEGF).
From: S1PR1 regulates the switch of two angiogenic modes by VE-cadherin phosphorylation in breast cancer
a Western blot showing that S1PR1 overexpression promoted phosphorylation of the Y731 site of VE-cadherin and β-catenin expression compared with that of the control groups. b VEGF protein expression was determined using an ELISA in the cell culture supernatant (OD 450 nm). S1PR1 overexpression increased VEGF secretion compared with that of the control groups. Downregulation of S1PR1 groups suppressed VEGF secretion. The mean ± SD is shown. *p < 0.05 (n = 3)
