Fig. 2: rhCXCL5 promotes HUVEC tube formation, migration, and proliferation through the CXCR2 pathway. | Cell Death & Disease

Fig. 2: rhCXCL5 promotes HUVEC tube formation, migration, and proliferation through the CXCR2 pathway.

From: CXCL5 induces tumor angiogenesis via enhancing the expression of FOXD1 mediated by the AKT/NF-κB pathway in colorectal cancer

Fig. 2

a Images of HUVEC tube formation assay in different groups. Formation of tube-like networks was stimulated by the addition of rhCXCL5. Number of tubes and total length of tubes demonstrate the ability of angiogenesis in each group. Scale bars, 200 μm (magnification ×40). b, c Number of tubes and total length of tubes significantly increase in the rhCXCL5 group and decrease in the CXCR2-shRNA group. d–f VEGF-A protein and mRNA expression were detected by ELISA, western blot, and qPCR in different groups. rhCXCL5 increases VEGF-A expression which is inhibited by CXCR2-shRNA. g Images of transwell assay in different groups. Scale bar, 100 μm (magnification ×100). h Migration cell numbers are increased in the rhCXCL5 group compared with rhCXCL5-stimulated HUVEC-shCXCR2 group and control group. i EdU assay results in different groups. Scale bar, 200 μm (magnification ×40). j Proportion of cells in the S phase markedly increase in the rhCXCL5 group compared with rhCXCL5-stimulated HUVEC-shCXCR2 group and control group. Data represent the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001

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