Fig. 1: MiR-15b and miR-322 were identified as potential miRNAs targeting the 3′-UTR of SETD3 gene.
From: MiR-15b and miR-322 inhibit SETD3 expression to repress muscle cell differentiation
a List the top 5 of predicted miRNAs targeting the 3′-UTR of mouse SETD3 gene. Score represents the potential capability of miRNA binding to 3′-UTR of SETD3 gene. b, c Dual-luciferase reporter assays were performed using the indicated miRNAs co-transfected with a dual-luciferase reporter construct containing either a long 3′-UTR fragment (756 bp, panel b) or a short 3′-UTR fragment (23 bp, panel c) of SETD3 gene. Dual-luciferase activities were normalized to the mock control, which was set as 1. Experiments were performed in three biological duplicates, and data are presented as mean ± SD. d Sequence alignment of mouse miR-15b or miR-322 with the 3′-UTR of SETD3 gene was shown. e Homologous comparisons of miR-15b or miR-322 in different species. mmu Mus musculus, hsa Homo sapiens, rno Rattus norvegicus
